Biopioneer Taq Polymerase Mat 1

Expand 20 kb plus pcr system.

Biopioneer taq polymerase mat 1. Dtt 25g from biopioneer 9. The mytaq one step rt pcr kit has been formulated for highly reproducible first strand cdna synthesis and subsequent pcr in a single tube. Highlights thermostable half life is more than 40 min at 95 c generates pcr products with 3 da overhangs supplied with two buffers 10x taq buffer with kcl and 10x taq buffer with nh 4 2 so 4. 0 5 u of taq dna polymerase lane 4.

Enzyme activity test of taq dna polymerase. 1 kb dna ladder cat. Taq dna polymerase was serially diluted from 5 u to 0 25 u and was used to amplify human genomic dna a and lambda dna b. Biopioneer taq possesses 5 3 exonuclease activity and leaves an a overhang such that the pcr product is suitable for effective integration into ta cloning vectors.

A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot start dna polymerase system ensures that mytaq one step rt pcr kit produces fast highly specific and ultra sensitive one step rt pcr perfect for all. Recombinant taq dna polymerase is the ideal tool for standard pcr of templates 5 kb or shorter. Optimizing taq polymerase concentration for improved signal to noise in the broad range detection of low abundance bacteria. Detection of the beta lactamase gene in commercial taq polymerase.

5 u of taq dna polymerase lane 2. Taq annealingdna polymerase recombinant lc component ep0403 ep0404 taq dna polymerase 1 u µl 100 u 500 u 10x taq buffer with kcl 0 6 ml2x1 25 10x taq buffer with nh 4 2 so 4 0 6 ml 2x1 25 ml 25 mm mgcl 2 0 6 ml 2x1 25 ml description taq dna polymerase is a highly thermostable dna polymerase of the thermophilic bacterium thermus. Thermostable dna polymerase mixture from roche applied science 5. Gel extraction kit from biopioneer 7.

Room temperature reaction setup using this master mix is fast and easy and simply pipet 12 5 ul hotstart taq master mix into each pcr tube and add 125. Mat 1 mat 1 new doc 59 kb download. The latter allows for pcr at wide range of magnesium concentrations and decreases unspecific priming. 2x hotstart taq super mix 1000 reactions hotstart taq master mix is a ready to use mixture of hotstart taq dna polymerase pcr buffer and dntps.

1 u of taq dna polymerase lane 3. 0 33 u of taq dna. Mouse anti bacteria rna polymerase sigma 70 monoclonal antibody unconjugated clone 2g10 from abr affinity bioreagents 6. Four dilutions of amplitaq dna polymerase were tested with primers for the beta.

D 1040 bioneer lane 1.